| Gene | Rnasek |
| Chromosome | 11 |
| Deletion Start | 70,239,730 |
| Deletion End | 70,238,249 |
| Deletion Size | 1,482 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,239,207 | 70,239,230 | CCTCCCTATACCCAGTCACTCTTC |
| TUP | 70,239,182 | 70,239,206 | CCAAGGTCCTACCTCACGCCCTGTT |
| TUR | 70,239,132 | 70,239,154 | GGGCTAGTTGTATCTGGTGGTTG |
| TDF | 70,238,486 | 70,238,505 | TGGCTGACATTTCCCTGCTC |
| TDP | 70,238,462 | 70,238,482 | CGCCACCTTCAGGAACGGTCC |
| TDR | 70,238,407 | 70,238,425 | CGCGGCGATGAAACAGTTG |
PCR Primers
| Primer | Sequence |
| SU | TAGCGTCTGCACCACTCAAC |
| SD | AGCTGGAAACCACGGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13778A-A10 | VGB6 | C57BL/6NTac | |
| 13778A-B8 | VGB6 | C57BL/6NTac | |
| 13778A-B9 | VGB6 | C57BL/6NTac | |
| 13778A-G3 | VGB6 | C57BL/6NTac | |
| 13778A-H6 | VGB6 | C57BL/6NTac | |
