13782   Rnf25     Status: ES cell colonies screened / QC positives

GeneRnf25
Chromosome1
Deletion Start74,601,362
Deletion End74,593,794
Deletion Size7,569
Genome Build38
MGI IDMGI:1890215
NCBI Gene ID57751
BAC ID224I14
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 1

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,601,26474,601,282TGCCGGGAACTAGCAACAG
TUP74,601,23574,601,257ACCGTCCGAACCACGGGCATAAA
TUR74,601,18674,601,207CTCGCCTACGAAAGGATGTCTC
TDF74,594,57874,594,597AGGCTGAACGGAACCGATAC
TDP74,594,54974,594,576TCATTAGCCTTCAGCAGGTGAGGAAAGG
TDR74,594,49874,594,517TAGGGCCACGGCATTCATAG

PCR Primers

PrimerSequence
SUCCCAGTCTAGACGCTCCAAG
SDGGCTGGAATCTGCTAGGAAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR97 bp++-
TDFTDR100 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13782A-A11VGB6C57BL/6NTac
13782A-D7VGB6C57BL/6NTac
13782A-G6VGB6C57BL/6NTac
13782A-G9VGB6C57BL/6NTac
13782A-H5VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image