| Gene | Rnf5 |
| Chromosome | 17 |
| Deletion Start | 34,603,470 |
| Deletion End | 34,601,471 |
| Deletion Size | 2,000 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,602,701 | 34,602,724 | TCTGGAGGATTAGGAGCAAGAATG |
| TUP | 34,602,668 | 34,602,696 | TCAGGCTGAAAGGAAACCTGTTGAATTGG |
| TUR | 34,602,647 | 34,602,667 | TCTGCCTCCGAACTTGACTTC |
| TDF | 34,602,132 | 34,602,150 | TGGCATCAGCAGGGAGAAG |
| TDP | 34,602,108 | 34,602,130 | TCGTCCCTCTTTATGGGCGAGGG |
| TDR | 34,602,029 | 34,602,049 | TGTCTCGGGATGGGACAGAAG |
PCR Primers
| Primer | Sequence |
| SU | CTGAGCCAATCCCTTCTGAG |
| SD | AAAGGAGGTAGTGAGGGATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 122 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13784A-A5 | VGB6 | C57BL/6NTac | |
| 13784A-G7 | VGB6 | C57BL/6NTac | |
| 13784A-B4 | VGB6 | C57BL/6NTac | |
| 13784A-C1 | VGB6 | C57BL/6NTac | |
| 13784A-F5 | VGB6 | C57BL/6NTac | |
| 13784A-A8 | VGB6 | C57BL/6NTac | |
