| Gene | Rnmtl1 |
| Chromosome | 11 |
| Deletion Start | 76,243,776 |
| Deletion End | 76,250,427 |
| Deletion Size | 6,652 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,244,404 | 76,244,423 | GCCGTCTGGAGTACGTCAAG |
| TUP | 76,244,428 | 76,244,453 | TGCCGGTAGACAAGCTGAAAGACGTC |
| TUR | 76,244,487 | 76,244,509 | GGCGTTACTAGGTCTGACCAATC |
| TDF | 76,250,163 | 76,250,183 | GCCCAAACTTGAGGTCCAGAG |
| TDP | 76,250,186 | 76,250,211 | ATGATTTGGACTGGACAGGAGCACCA |
| TDR | 76,250,254 | 76,250,272 | CGGCCAGTTGCAGGGATTC |
PCR Primers
| Primer | Sequence |
| SU | ACGTGCGTAGACAACACAGC |
| SD | AATAAGGAACAGCAGACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13785A-E7 | VGB6 | C57BL/6NTac | |
| 13785B-G10 | VGB6 | C57BL/6NTac | |
