| Gene | Rpl10a |
| Chromosome | 17 |
| Deletion Start | 28,328,617 |
| Deletion End | 28,331,065 |
| Deletion Size | 2,449 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,329,008 | 28,329,028 | ACGACCCTCAGAAGGACAAAC |
| TUP | 28,329,030 | 28,329,052 | TTTCTCGGGCACCGTCAGGTTGG |
| TUR | 28,329,078 | 28,329,096 | AGGCCACACGGGAACACTG |
| TDF | 28,330,261 | 28,330,282 | CCCTCAGTTCTATGCAGCACAC |
| TDP | 28,330,283 | 28,330,309 | CAGGGATTGTGGGACATCCTGGTCTAA |
| TDR | 28,330,315 | 28,330,334 | CCACCCTCAAGTCCTCACAG |
PCR Primers
| Primer | Sequence |
| SU | AGAAGATAGAAAGCAGGGAG |
| SD | ACCTCTGTCACCCACAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13788A-A9 | VGB6 | C57BL/6NTac | |
| 13788A-C1 | VGB6 | C57BL/6NTac | |
| 13788A-C10 | VGB6 | C57BL/6NTac | |
| 13788A-F9 | VGB6 | C57BL/6NTac | |
| 13788A-E6 | VGB6 | C57BL/6NTac | |
| 13788A-D8 | VGB6 | C57BL/6NTac | |
