| Gene | Rps27a |
| Chromosome | 11 |
| Deletion Start | 29,547,725 |
| Deletion End | 29,545,813 |
| Deletion Size | 1,913 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,546,962 | 29,546,979 | CAATGGCCCAGTCCACAC |
| TUP | 29,546,929 | 29,546,955 | CTCAACACTAAGGAGGTAGAGGCAGCC |
| TUR | 29,546,905 | 29,546,926 | GCCCTGGAACTTTCTCTGTAAC |
| TDF | 29,546,549 | 29,546,567 | GCTCAGGGCCAACTTGAAC |
| TDP | 29,546,514 | 29,546,543 | TCCTGAGCCTCCTTAAAATTCCTAGTGCTG |
| TDR | 29,546,495 | 29,546,513 | GGTGTTGGGCCTGTCACTC |
PCR Primers
| Primer | Sequence |
| SU | ATCGGCTGTATAAGAAAGCC |
| SD | CCACCATGCCTAGCTTGTATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13803C-B10 | VGB6 | C57BL/6NTac | |
| 13803D-A12 | VGB6 | C57BL/6NTac | |
