| Gene | Sac3d1 |
| Chromosome | 19 |
| Deletion Start | 6,118,583 |
| Deletion End | 6,116,012 |
| Deletion Size | 2,572 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,117,807 | 6,117,824 | CAGGGCCTCTTTCTGCTC |
| TUP | 6,117,781 | 6,117,805 | ACAACTTGGGTGAGTCGATCCCAGA |
| TUR | 6,117,713 | 6,117,732 | TAGTTCGCCGTTGCTGCTAC |
| TDF | 6,116,436 | 6,116,457 | TCGCACCTTACCCTACCTACAG |
| TDP | 6,116,410 | 6,116,432 | TGCGCAGTGCAGGAACACATTGG |
| TDR | 6,116,378 | 6,116,397 | ACAGGCGGGCTAGAGCTTTG |
PCR Primers
| Primer | Sequence |
| SU | AAGAATGACAGGCACGCAG |
| SD | TTTCCTGAGGCCAGGAGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 112 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13808A-B8 | VGB6 | C57BL/6NTac | |
| 13808A-C7 | VGB6 | C57BL/6NTac | |
| 13808A-H11 | VGB6 | C57BL/6NTac | |
| 13808A-G7 | VGB6 | C57BL/6NTac | |
| 13808A-G8 | VGB6 | C57BL/6NTac | |
| 13808A-F8 | VGB6 | C57BL/6NTac | |
