| Gene | Sap18 |
| Chromosome | 14 |
| Deletion Start | 57,798,286 |
| Deletion End | 57,802,147 |
| Deletion Size | 3,862 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,798,927 | 57,798,945 | GGTGTCAAAGCGTTTGGTG |
| TUP | 57,798,955 | 57,798,977 | TGAGGACGGCTGTCCCAAGTGTT |
| TUR | 57,798,979 | 57,798,999 | CAAGGCAGGTACTCGGAGATG |
| TDF | 57,801,325 | 57,801,344 | GACACAGGTTGCCAGGATTG |
| TDP | 57,801,348 | 57,801,372 | AGCAAACTGACTTCCCTGCCAGGCC |
| TDR | 57,801,380 | 57,801,402 | CCAGTGCTGCACACTATATGATG |
PCR Primers
| Primer | Sequence |
| SU | TACGTTAAGCTGACCTGGGC |
| SD | TGCCACAAACATATTCACCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13810A-B12 | VGB6 | C57BL/6NTac | |
| 13810A-A1 | VGB6 | C57BL/6NTac | |
| 13810A-F7 | VGB6 | C57BL/6NTac | |
| 13810A-E10 | VGB6 | C57BL/6NTac | |
| 13810A-E4 | VGB6 | C57BL/6NTac | |
| 13810A-D3 | VGB6 | C57BL/6NTac | |
