| Gene | Sart1 |
| Chromosome | 19 |
| Deletion Start | 5,388,624 |
| Deletion End | 5,380,028 |
| Deletion Size | 8,597 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,388,137 | 5,388,154 | CTTCCTGCCTTCCCTGTC |
| TUP | 5,388,113 | 5,388,136 | TTCCTGGCACTTCACAGCAATGCA |
| TUR | 5,388,083 | 5,388,102 | ACGCTCGTCCCACCTCATTC |
| TDF | 5,380,531 | 5,380,552 | CCTCTGCTCCTGCTCTGTATCC |
| TDP | 5,380,508 | 5,380,529 | AGGCATTCCGGCAGCTGTCCCA |
| TDR | 5,380,475 | 5,380,496 | TTCATCTTGCCTGAGCCCTTTC |
PCR Primers
| Primer | Sequence |
| SU | TAGCTCCGGAGATGATGACC |
| SD | GTGACAGGTACACGCCACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13813A-B3 | VGB6 | C57BL/6NTac | |
| 13813A-H4 | VGB6 | C57BL/6NTac | |
| 13813A-C2 | VGB6 | C57BL/6NTac | |
| 13813A-D4 | VGB6 | C57BL/6NTac | |
| 13813A-E11 | VGB6 | C57BL/6NTac | |
| 13813A-D6 | VGB6 | C57BL/6NTac | |
