| Gene | Scgb1c1 |
| Chromosome | 7 |
| Deletion Start | 140,845,580 |
| Deletion End | 140,846,650 |
| Deletion Size | 1,071 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 140,845,618 | 140,845,636 | GCTCTGCGTCTGTGGTAAG |
| TUP | 140,845,637 | 140,845,663 | TCTGGGCCTTGTTTCTAGCACTTTCGG |
| TUR | 140,845,678 | 140,845,698 | TCCCAGGATTTCAGCCTCTTG |
| TDF | 140,846,216 | 140,846,238 | CTGTGCATAAGGAGCAACTGGTC |
| TDP | 140,846,245 | 140,846,270 | CTGGTAATCGGGCTGGGACTCCCATC |
| TDR | 140,846,278 | 140,846,299 | GGGTCTCGGAAGAACTGCAAAC |
PCR Primers
| Primer | Sequence |
| SU | AGGTCACACATGACCTTTGG |
| SD | CATTGCTTCCGGAAAGTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13814A-B3 | VGB6 | C57BL/6NTac | |
| 13814A-B4 | VGB6 | C57BL/6NTac | |
| 13814A-D5 | VGB6 | C57BL/6NTac | |
| 13814A-F1 | VGB6 | C57BL/6NTac | |
| 13814A-E11 | VGB6 | C57BL/6NTac | |
| 13814A-E12 | VGB6 | C57BL/6NTac | |
