| Gene | Scn8a |
| Chromosome | 15 |
| Deletion Start | 100,936,328 |
| Deletion End | 100,972,954 |
| Deletion Size | 36,627 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,936,454 | 100,936,472 | CACCGGGAGGACGATGAAG |
| TUP | 100,936,473 | 100,936,497 | ACAGCAAGCCCAAGCCAAACAGTGA |
| TUR | 100,936,513 | 100,936,534 | CCCGTAGATGAAAGGCAAACTC |
| TDF | 100,972,852 | 100,972,873 | CATGGCTTACGAAGAGCAGAAC |
| TDP | 100,972,878 | 100,972,903 | CAACACTGGAGGAGGCAGAGCAGAAA |
| TDR | 100,972,912 | 100,972,930 | CTCCAGCATCGCCTTGAAC |
PCR Primers
| Primer | Sequence |
| SU | TTGGAGCGTAGATCCAGAGG |
| SD | TCCCAGTGTTTCTCTAATCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13815A-D5 | VGB6 | C57BL/6NTac | |
| 13815A-H3 | VGB6 | C57BL/6NTac | |
