| Gene | Sdf2l1 |
| Chromosome | 16 |
| Deletion Start | 17,132,307 |
| Deletion End | 17,130,502 |
| Deletion Size | 1,806 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,131,621 | 17,131,640 | ACCTGCACACGCACCACTTC |
| TUP | 17,131,596 | 17,131,619 | CGTCGCCGCTATCCAACAACCAGG |
| TUR | 17,131,563 | 17,131,582 | ACGCAGCCTCTGTGGGTTTC |
| TDF | 17,131,264 | 17,131,284 | CCTCTGCGAAAGAGTTCCTAC |
| TDP | 17,131,232 | 17,131,258 | TACAGTGTTCATTGCAGCTGGTCTGGG |
| TDR | 17,131,196 | 17,131,218 | CTCTCCAAAGCACTGTCAGATTC |
PCR Primers
| Primer | Sequence |
| SU | TGACTAGAAGCAACCAACCC |
| SD | CAGGTGGCAACAAACACATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13819A-A2 | VGB6 | C57BL/6NTac | |
| 13819A-B3 | VGB6 | C57BL/6NTac | |
| 13819A-B5 | VGB6 | C57BL/6NTac | |
| 13819A-C2 | VGB6 | C57BL/6NTac | |
| 13819A-D6 | VGB6 | C57BL/6NTac | |
| 13819A-D4 | VGB6 | C57BL/6NTac | |
