| Gene | Sec11a |
| Chromosome | 7 |
| Deletion Start | 80,947,449 |
| Deletion End | 80,915,794 |
| Deletion Size | 31,656 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,947,116 | 80,947,137 | GCGCTTTGTAAGTGGGAGTTTG |
| TUP | 80,947,084 | 80,947,112 | CACTGTAAAGCGGCAGACTTAACCGTTTC |
| TUR | 80,947,042 | 80,947,062 | GCTCCTGTGGGACCTGTAATG |
| TDF | 80,916,756 | 80,916,776 | GGAGACTACAGCAGGTGGAAG |
| TDP | 80,916,732 | 80,916,755 | TAAGCTCAAGGCAAGCCTGGGCTG |
| TDR | 80,916,678 | 80,916,698 | CTGGCTTTGGCCCTCTTACTC |
PCR Primers
| Primer | Sequence |
| SU | CTAGGGTCACTGTCTTCGGG |
| SD | GCCTCAATATGTGCCATTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13820A-H3 | VGB6 | C57BL/6NTac | |
| 13820A-G6 | VGB6 | C57BL/6NTac | |
| 13820A-B12 | VGB6 | C57BL/6NTac | |
| 13820A-A12 | VGB6 | C57BL/6NTac | |
| 13820A-A11 | VGB6 | C57BL/6NTac | |
| 13820A-F10 | VGB6 | C57BL/6NTac | |
