| Gene | Sema3b |
| Chromosome | 9 |
| Deletion Start | 107,605,074 |
| Deletion End | 107,598,755 |
| Deletion Size | 6,320 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,604,508 | 107,604,530 | GATGGATTCTGTCCTGTCAGTTG |
| TUP | 107,604,475 | 107,604,500 | TTTGCCTCTCCTAGAACCCTGCTGTC |
| TUR | 107,604,455 | 107,604,474 | TGCTGGAAGGCAGGCTTATG |
| TDF | 107,598,832 | 107,598,852 | ACGTCGTAAGGGTCGCAACAG |
| TDP | 107,598,807 | 107,598,830 | CGGATGCATGTCTCTGAGCTCCGT |
| TDR | 107,598,778 | 107,598,796 | TGAGCTGCACTACGTGGTC |
PCR Primers
| Primer | Sequence |
| SU | TTACTGAGGGTGACGTGGTG |
| SD | GTACAGTTGGCCTGCCTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13823C-C4 | VGB6 | C57BL/6NTac | |
| 13823C-C10 | VGB6 | C57BL/6NTac | |
| 13823C-D10 | VGB6 | C57BL/6NTac | |
| 13823C-D11 | VGB6 | C57BL/6NTac | |
