| Gene | Sharpin |
| Chromosome | 15 |
| Deletion Start | 76,350,675 |
| Deletion End | 76,347,182 |
| Deletion Size | 3,494 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,350,216 | 76,350,240 | TCCCTGTTCAGGTCAGTTTAGAATG |
| TUP | 76,350,185 | 76,350,210 | TGGAGGCTATTTGTTACACCGTCCGT |
| TUR | 76,350,162 | 76,350,180 | TGCAGCTCGTGTTGGTTAG |
| TDF | 76,347,355 | 76,347,374 | GCCCTTCCTGCACTTTCATC |
| TDP | 76,347,331 | 76,347,353 | ATGCCTCAAACCGCCCTGGCTGT |
| TDR | 76,347,309 | 76,347,328 | GCCTTTGGGTGCTACACATC |
PCR Primers
| Primer | Sequence |
| SU | TGTCTTGTGTAGGGCAGCAG |
| SD | CCTAGCAGGAGGCTAGAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13829A-A10 | VGB6 | C57BL/6NTac | |
| 13829A-D2 | VGB6 | C57BL/6NTac | |
| 13829A-F4 | VGB6 | C57BL/6NTac | |
| 13829A-F10 | VGB6 | C57BL/6NTac | |
| 13829A-G10 | VGB6 | C57BL/6NTac | |
