| Gene | Shf |
| Chromosome | 2 |
| Deletion Start | 122,359,478 |
| Deletion End | 122,349,352 |
| Deletion Size | 10,127 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 122,359,046 | 122,359,064 | TGAGTCAGACCTGCCCTTG |
| TUP | 122,359,016 | 122,359,042 | AAACTGAGGCCAAGAGATGACCCAGGG |
| TUR | 122,358,976 | 122,358,999 | CCTCTTCTATCCTCTCCATTCACC |
| TDF | 122,350,074 | 122,350,093 | TGAGAGGGTCTGACTTGGTG |
| TDP | 122,350,050 | 122,350,072 | AAGACCCACAGACTGGTGCTGCC |
| TDR | 122,350,012 | 122,350,032 | CCCGGCCTTATGCTCAGTTTC |
PCR Primers
| Primer | Sequence |
| SU | TGTCCAGCTTGGACCCTTAC |
| SD | CAATTTATAGTCGGGCTCGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13830D-B11 | VGB6 | C57BL/6NTac | |
| 13830D-D1 | VGB6 | C57BL/6NTac | |
| 13830D-E9 | VGB6 | C57BL/6NTac | |
| 13830D-G2 | VGB6 | C57BL/6NTac | |
| 13830D-G4 | VGB6 | C57BL/6NTac | |
