| Gene | Slc16a11 |
| Chromosome | 11 |
| Deletion Start | 70,214,514 |
| Deletion End | 70,216,439 |
| Deletion Size | 1,926 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,214,755 | 70,214,775 | GAGGCCGTGGATGTAGTGATG |
| TUP | 70,214,781 | 70,214,810 | CAGCAGACTTGATTACCAGACAACCTGAGA |
| TUR | 70,214,843 | 70,214,863 | TCCTCCCATTGACCTCAAGAC |
| TDF | 70,215,701 | 70,215,720 | CAGCGTTAGTGGTGGCTGTC |
| TDP | 70,215,728 | 70,215,749 | TGGGAGATGCCTGTGCCCGATT |
| TDR | 70,215,805 | 70,215,825 | CCCAGTCAGAGACCCAAACAC |
PCR Primers
| Primer | Sequence |
| SU | AGTTGGGCTCCATCTCTGTG |
| SD | GGTTGTCCTCCACAAACACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 125 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13832A-A3 | VGB6 | C57BL/6NTac | |
| 13832A-A11 | VGB6 | C57BL/6NTac | |
| 13832A-F9 | VGB6 | C57BL/6NTac | |
| 13832A-G12 | VGB6 | C57BL/6NTac | |
| 13832A-H2 | VGB6 | C57BL/6NTac | |
| 13832A-E12 | VGB6 | C57BL/6NTac | |
