| Gene | Slc16a3 |
| Chromosome | 11 |
| Deletion Start | 120,955,250 |
| Deletion End | 120,958,164 |
| Deletion Size | 2,915 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,955,599 | 120,955,619 | TCAGGTTCCACAAGCAGTTTC |
| TUP | 120,955,621 | 120,955,644 | TCAAAGGTGCTTGATGCCTGCTGC |
| TUR | 120,955,659 | 120,955,677 | GCATGGCTCAGCTCCTCAC |
| TDF | 120,957,742 | 120,957,762 | CAGGGTGACTGCATGTCTGAG |
| TDP | 120,957,767 | 120,957,791 | TTCCTCAATGCCAGGAGATGGAGCC |
| TDR | 120,957,844 | 120,957,863 | GCAGCTTGCCTGCAAAGAAG |
PCR Primers
| Primer | Sequence |
| SU | GCGAGAGTTCAGAAGATGCC |
| SD | CCCTGCCCTCTGATTAAAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 122 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13833A-A8 | VGB6 | C57BL/6NTac | |
| 13833A-H6 | VGB6 | C57BL/6NTac | |
| 13833A-H4 | VGB6 | C57BL/6NTac | |
| 13833A-G8 | VGB6 | C57BL/6NTac | |
| 13833A-E7 | VGB6 | C57BL/6NTac | |
| 13833A-E3 | VGB6 | C57BL/6NTac | |
