13834   Slc25a35     Status: ES cell colonies screened / QC positives

GeneSlc25a35
Chromosome11
Deletion Start68,968,666
Deletion End68,972,146
Deletion Size3,481
Genome Build38
MGI IDMGI:1919248
NCBI Gene ID71998
BAC ID439E20
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF68,968,86768,968,887GCGCTCTTGTACCAGTTCTTG
TUP68,968,88968,968,912TGAATGGCATCCGACTGGGCACCT
TUR68,968,94468,968,965ACTGTGGGTGCCTTCATTAGTG
TDF68,971,25768,971,276GCCTGCCCAGAGTTGTCATC
TDP68,971,27968,971,303CTCCTCTACCCAGCTGTGCACCTTC
TDR68,971,32168,971,340GTACCTCCCACTGGCTCAAG

PCR Primers

PrimerSequence
SUCTTTCACAACCCTCCTCTCG
SDTCTTTCTGCTTCTTCCCTGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR99 bp++-
TDFTDR84 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13834A-A6VGB6C57BL/6NTac
13834A-A11VGB6C57BL/6NTac
13834A-G12VGB6C57BL/6NTac
13834A-D9VGB6C57BL/6NTac
13834A-G6VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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