| Gene | Slc35e3 |
| Chromosome | 10 |
| Deletion Start | 117,746,183 |
| Deletion End | 117,736,030 |
| Deletion Size | 10,154 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 117,745,458 | 117,745,478 | GCGAAAGCTCAACCTGGAAAG |
| TUP | 117,745,423 | 117,745,449 | ATCCCCAGGAAATCGAACCAGAAGTGA |
| TUR | 117,745,368 | 117,745,389 | GTGTGCCACTGAAGGTGTATTC |
| TDF | 117,736,644 | 117,736,667 | GCAGGTCCAACAGGAAAGAATAAG |
| TDP | 117,736,618 | 117,736,643 | TATGGTGTTGGGCTCAGCAGTCAAGA |
| TDR | 117,736,596 | 117,736,616 | CCTCTGTAAGAGCAGGGTGTG |
PCR Primers
| Primer | Sequence |
| SU | GCTCAGCAACGTGGAGAGT |
| SD | TCATTCCTATGGAGCCGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 111 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13837A-E7 | VGB6 | C57BL/6NTac | |
| 13837A-D3 | VGB6 | C57BL/6NTac | |
