| Gene | Sox8 |
| Chromosome | 17 |
| Deletion Start | 25,570,519 |
| Deletion End | 25,567,282 |
| Deletion Size | 3,238 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,570,151 | 25,570,169 | GGACCAGTACCCGCATCTC |
| TUP | 25,570,126 | 25,570,150 | CATAACGCAGAGCTCAGCAAGACCC |
| TUR | 25,570,078 | 25,570,098 | TCAGCCTGAGTGTCAAGAGTC |
| TDF | 25,567,702 | 25,567,719 | TCAGCTTCGCCCACAGAG |
| TDP | 25,567,671 | 25,567,694 | CCCTTCGGCCACAGATCAAGACGG |
| TDR | 25,567,630 | 25,567,651 | GCCATGTGACTGGTCGTTGTAG |
PCR Primers
| Primer | Sequence |
| SU | GCAAGGCTTCTTTGGATTCG |
| SD | ATTGGTCCCAAGAGAGTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13839A-B1 | VGB6 | C57BL/6NTac | |
| 13839A-G3 | VGB6 | C57BL/6NTac | |
| 13839A-G11 | VGB6 | C57BL/6NTac | |
