| Gene | Slc36a3 |
| Chromosome | 11 |
| Deletion Start | 55,151,318 |
| Deletion End | 55,124,873 |
| Deletion Size | 26,446 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,150,955 | 55,150,978 | GCCTGAGCTACTGAACACAAAGAG |
| TUP | 55,150,925 | 55,150,949 | CCCTGACACCAGGAGGATGGATGCT |
| TUR | 55,150,888 | 55,150,910 | GGGAGATGGACTGGGATAGAGAC |
| TDF | 55,125,709 | 55,125,729 | TTCACCTATGCCCTCCAGTTC |
| TDP | 55,125,684 | 55,125,708 | CACGTCCCAGCTGAGATCATCGTCC |
| TDR | 55,125,648 | 55,125,666 | GAGCCCAGTTCTCAGACAC |
PCR Primers
| Primer | Sequence |
| SU | GACTGATCTGGGAGAGGCTG |
| SD | TTTGAGGCTGATAGTATCAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13840A-E4 | VGB6 | C57BL/6NTac | |
| 13840A-D6 | VGB6 | C57BL/6NTac | |
