| Gene | Slc4a1ap |
| Chromosome | 5 |
| Deletion Start | 31,526,986 |
| Deletion End | 31,553,785 |
| Deletion Size | 26,800 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,527,133 | 31,527,151 | TCCAGTCCCTCCGTTTCTG |
| TUP | 31,527,158 | 31,527,183 | TGAAGAAAGAATGTCTCGCGGCGCAG |
| TUR | 31,527,194 | 31,527,212 | ACCTCGGGCTCCTCACATC |
| TDF | 31,553,155 | 31,553,176 | ACACCAGGTACAGAGACACATC |
| TDP | 31,553,179 | 31,553,205 | TGGGAGTGCTGACAATAACATCCTGGG |
| TDR | 31,553,221 | 31,553,242 | GCATATCCTTAGCGTGCCATTC |
PCR Primers
| Primer | Sequence |
| SU | GATTAATGGACCAAGGCCAC |
| SD | ACTCACCCTGAGTGGCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13843A-H3 | VGB6 | C57BL/6NTac | |
| 13843A-H8 | VGB6 | C57BL/6NTac | |
