| Gene | Ephx3 |
| Chromosome | 17 |
| Deletion Start | 32,189,445 |
| Deletion End | 32,184,889 |
| Deletion Size | 4,557 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,188,631 | 32,188,651 | CAGAGCAGAAAGGTGGGATGG |
| TUP | 32,188,603 | 32,188,630 | CCCAAAGCTGGGTATAGGAGGTGCATTC |
| TUR | 32,188,575 | 32,188,598 | GGTAAGGGAGTTGGGAGTTTAGTC |
| TDF | 32,185,509 | 32,185,530 | CGGCCAGATATTCCGATCAAAC |
| TDP | 32,185,481 | 32,185,502 | TTCCTGTTCCAGCTTCCCTGGC |
| TDR | 32,185,441 | 32,185,462 | TGCACCTGGAAGTCAGACATAG |
PCR Primers
| Primer | Sequence |
| SU | TGCTGCAGTATATGTGTGAACAG |
| SD | TCCTGGCTTCAGAGACATTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13845A-D4 | VGB6 | C57BL/6NTac | |
| 13845A-G12 | VGB6 | C57BL/6NTac | |
| 13845A-F2 | VGB6 | C57BL/6NTac | |
| 13845A-F12 | VGB6 | C57BL/6NTac | |
| 13845A-F8 | VGB6 | C57BL/6NTac | |
