| Gene | Slc4a9 |
| Chromosome | 18 |
| Deletion Start | 36,528,190 |
| Deletion End | 36,541,318 |
| Deletion Size | 13,129 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,528,567 | 36,528,584 | GCAGCCACATTGCCCTAC |
| TUP | 36,528,586 | 36,528,611 | TGTGTTCCAGACATGGGCGAGAGTCT |
| TUR | 36,528,632 | 36,528,650 | GCAGCCACCTGTGATGAAG |
| TDF | 36,540,709 | 36,540,729 | CTCCAGGGCTTGCTTCTGTTC |
| TDP | 36,540,733 | 36,540,754 | CAGTTACTGGGCCTGGTGGCCA |
| TDR | 36,540,775 | 36,540,796 | GGAGTTCCTGTGGTGAGAAGAC |
PCR Primers
| Primer | Sequence |
| SU | ACCAAACAGTCCTGGATTCG |
| SD | TCCGAAGTGTTACATGGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13846A-B2 | VGB6 | C57BL/6NTac | |
| 13846A-B11 | VGB6 | C57BL/6NTac | |
| 13846A-C4 | VGB6 | C57BL/6NTac | |
| 13846A-E6 | VGB6 | C57BL/6NTac | |
| 13846A-H8 | VGB6 | C57BL/6NTac | |
| 13846A-H9 | VGB6 | C57BL/6NTac | |
