| Gene | Skiv2l |
| Chromosome | 17 |
| Deletion Start | 34,850,152 |
| Deletion End | 34,839,376 |
| Deletion Size | 10,777 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,849,862 | 34,849,883 | TGACCCTCACCTTTGACCTATG |
| TUP | 34,849,827 | 34,849,855 | AAAGAAATTCCACCCTGCTCACAGGAGGA |
| TUR | 34,849,794 | 34,849,812 | AGCCGGAGGCCAAGATGAC |
| TDF | 34,840,325 | 34,840,344 | GTCTGACCAGTCGCTGTTAC |
| TDP | 34,840,303 | 34,840,324 | TGCTCCCTGAGTACCACCAGCG |
| TDR | 34,840,280 | 34,840,301 | CATCCTGCCCACTTACCTCTAC |
PCR Primers
| Primer | Sequence |
| SU | AGACGAAGCCCTCACCTGA |
| SD | GGCTCAATCTCTGGCTTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13851A-F2 | VGB6 | C57BL/6NTac | |
| 13851A-C11 | VGB6 | C57BL/6NTac | |
| 13851A-A8 | VGB6 | C57BL/6NTac | |
