| Gene | Smpd2 |
| Chromosome | 10 |
| Deletion Start | 41,490,029 |
| Deletion End | 41,487,111 |
| Deletion Size | 2,919 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,489,245 | 41,489,266 | CCTGTGTTCTCAGCCTGTTCTC |
| TUP | 41,489,212 | 41,489,239 | AGGAAGCTCTCCAACATTCTTGCTTGCA |
| TUR | 41,489,169 | 41,489,186 | TGACGGCCTGCTCTTCTC |
| TDF | 41,487,953 | 41,487,975 | GGTGTGCCTTCTCCAGTATCTTC |
| TDP | 41,487,926 | 41,487,950 | TCCTCTGTCACTAGCCCACGCTTTA |
| TDR | 41,487,871 | 41,487,891 | CCAGCCGACAGGATTCTATTC |
PCR Primers
| Primer | Sequence |
| SU | AACTTCAAGCCTGTTGCTGG |
| SD | TCTGATCCAATTCCAGGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13852A-A10 | VGB6 | C57BL/6NTac | |
| 13852A-F11 | VGB6 | C57BL/6NTac | |
| 13852A-E12 | VGB6 | C57BL/6NTac | |
| 13852A-E9 | VGB6 | C57BL/6NTac | |
| 13852A-C11 | VGB6 | C57BL/6NTac | |
