| Gene | Sox15 |
| Chromosome | 11 |
| Deletion Start | 69,655,376 |
| Deletion End | 69,656,663 |
| Deletion Size | 1,288 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,655,632 | 69,655,651 | TGCTGGGCGATGAAGAGAAG |
| TUP | 69,655,654 | 69,655,678 | ACCCTTCGTGGAGGAGGCTAAGCGT |
| TUR | 69,655,700 | 69,655,722 | CGGTACTTGTAGTCGGGATAGTC |
| TDF | 69,656,036 | 69,656,056 | GGCCCAGAGTTCAGGATAGTG |
| TDP | 69,656,063 | 69,656,092 | CCATCTCAACTAATGTGTCCTACCTCTGCC |
| TDR | 69,656,105 | 69,656,123 | GCCAGCCACAAGGAGAAAC |
PCR Primers
| Primer | Sequence |
| SU | TGAGTACAGGTGGGAAAGGC |
| SD | CTCTGGCCCTGTCCACTTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13857A-H12 | VGB6 | C57BL/6NTac | |
| 13857A-E11 | VGB6 | C57BL/6NTac | |
| 13857A-F12 | VGB6 | C57BL/6NTac | |
| 13857A-G12 | VGB6 | C57BL/6NTac | |
| 13857A-C1 | VGB6 | C57BL/6NTac | |
