| Gene | Spcs1 |
| Chromosome | 14 |
| Deletion Start | 31,001,237 |
| Deletion End | 31,000,029 |
| Deletion Size | 1,209 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,000,990 | 31,001,009 | GGCCCTCCTGGTGTTGAATG |
| TUP | 31,000,963 | 31,000,989 | CTGCCTTATGCCAACGCTGAGTTCCCT |
| TUR | 31,000,926 | 31,000,947 | CGAGTTGGTCCTACTGCCAAAG |
| TDF | 31,000,210 | 31,000,232 | TCTGATCTAGCTGACACTTCCTC |
| TDP | 31,000,188 | 31,000,209 | CATGGCCTATCTACCGCCGCCA |
| TDR | 31,000,152 | 31,000,175 | GTGCCTAAGTCTTGAACAGGTAAC |
PCR Primers
| Primer | Sequence |
| SU | GAGTAAAGGCCGTCGCTATG |
| SD | CACACGGGAAAGAAGCATTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13858A-B4 | VGB6 | C57BL/6NTac | |
| 13858A-B11 | VGB6 | C57BL/6NTac | |
| 13858A-F12 | VGB6 | C57BL/6NTac | |
| 13858A-C12 | VGB6 | C57BL/6NTac | |
| 13858A-H1 | VGB6 | C57BL/6NTac | |
