| Gene | Srp9 |
| Chromosome | 1 |
| Deletion Start | 182,124,907 |
| Deletion End | 182,131,471 |
| Deletion Size | 6,565 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 182,125,266 | 182,125,288 | CCTGTCCGTCGCTTAGTTTAGTG |
| TUP | 182,125,291 | 182,125,315 | CCTTTCCTTTCCTACCCAGACCCGA |
| TUR | 182,125,336 | 182,125,356 | AACTGGGACGTACAGTAGCAC |
| TDF | 182,131,011 | 182,131,031 | TGCCTTAGCAAGCCTGAAGAG |
| TDP | 182,131,034 | 182,131,054 | CGCCAGTTCCCTGGAGCTGGA |
| TDR | 182,131,068 | 182,131,091 | TGCAAGTTAAGTCCCACTGTCTAC |
PCR Primers
| Primer | Sequence |
| SU | CTTTGAGTGTGACAGCTGCG |
| SD | CTCTTCACACCACAGGATCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13862A-C11 | VGB6 | C57BL/6NTac | |
| 13862A-D8 | VGB6 | C57BL/6NTac | |
| 13862A-E5 | VGB6 | C57BL/6NTac | |
| 13862A-E11 | VGB6 | C57BL/6NTac | |
| 13862A-F5 | VGB6 | C57BL/6NTac | |
| 13862A-G6 | VGB6 | C57BL/6NTac | |
