| Gene | Stk16 |
| Chromosome | 1 |
| Deletion Start | 75,211,332 |
| Deletion End | 75,214,021 |
| Deletion Size | 2,690 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,211,949 | 75,211,968 | GGAGGGCTTGCATGATGGAC |
| TUP | 75,211,971 | 75,211,994 | TTCTACGCCCTGAAGCGGATCCTG |
| TUR | 75,212,016 | 75,212,035 | GCCTCTCGTTGGGCTTCTTC |
| TDF | 75,213,630 | 75,213,651 | GTGTTCCAGAAGGGTGACAGTG |
| TDP | 75,213,652 | 75,213,673 | TGGCCCTTGCTGTGCAGAATGA |
| TDR | 75,213,675 | 75,213,694 | GGGCTTTGTGGGATGCTGAG |
PCR Primers
| Primer | Sequence |
| SU | TTGTAGTGGCAGACCCTGTG |
| SD | TTGGCACAGACAGAAACCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13867A-D9 | VGB6 | C57BL/6NTac | |
| 13867A-G11 | VGB6 | C57BL/6NTac | |
| 13867A-C3 | VGB6 | C57BL/6NTac | |
| 13867A-C5 | VGB6 | C57BL/6NTac | |
| 13867A-F1 | VGB6 | C57BL/6NTac | |
| 13867A-D12 | VGB6 | C57BL/6NTac | |
