| Gene | Stub1 |
| Chromosome | 17 |
| Deletion Start | 25,832,850 |
| Deletion End | 25,830,786 |
| Deletion Size | 2,065 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,832,250 | 25,832,268 | TTGACGCCTGGTCTTGGTC |
| TUP | 25,832,225 | 25,832,248 | CCAGACTCGGAACCCACTTGTGGC |
| TUR | 25,832,175 | 25,832,197 | CAGGCTGCTGCATCTTCAGATAG |
| TDF | 25,830,884 | 25,830,905 | GGCTGGGTAGAGGACTATTGAG |
| TDP | 25,830,860 | 25,830,880 | CCATGTCCTGCCTGGCACCCT |
| TDR | 25,830,802 | 25,830,824 | GCCCAGGGACACAAACTATACAG |
PCR Primers
| Primer | Sequence |
| SU | AAGGCCACTTCTCAATGACC |
| SD | ATCCAGGCTGGTGAAGTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13868A-H10 | VGB6 | C57BL/6NTac | |
| 13868A-F1 | VGB6 | C57BL/6NTac | |
| 13868A-C1 | VGB6 | C57BL/6NTac | |
