| Gene | Surf2 |
| Chromosome | 2 |
| Deletion Start | 26,916,429 |
| Deletion End | 26,919,831 |
| Deletion Size | 3,403 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,916,438 | 26,916,461 | CCCTCTGATGTGCTTGCATTCTTG |
| TUP | 26,916,471 | 26,916,495 | CCCAGCTTGAGACTACTGCCCAACA |
| TUR | 26,916,508 | 26,916,527 | TTCCGGTCACGGTTCTCTTC |
| TDF | 26,919,023 | 26,919,043 | TGAGTGACGACAGCATGACAG |
| TDP | 26,919,045 | 26,919,072 | CCTATACCCACGTAAGCAGACCCAGTCC |
| TDR | 26,919,102 | 26,919,122 | GGCTCACAGCAGGATCACAAG |
PCR Primers
| Primer | Sequence |
| SU | GGGCTAAATCCAGGGAACC |
| SD | AAGATGCTTTAGCTTTATGTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13869A-B3 | VGB6 | C57BL/6NTac | |
| 13869A-C11 | VGB6 | C57BL/6NTac | |
| 13869A-E5 | VGB6 | C57BL/6NTac | |
| 13869A-E7 | VGB6 | C57BL/6NTac | |
| 13869A-H10 | VGB6 | C57BL/6NTac | |
| 13869A-H9 | VGB6 | C57BL/6NTac | |
