| Gene | Pate4 |
| Chromosome | 9 |
| Deletion Start | 35,611,843 |
| Deletion End | 35,607,685 |
| Deletion Size | 4,159 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,611,808 | 35,611,827 | TCAGCACACTGCTCATCGTG |
| TUP | 35,611,767 | 35,611,795 | CTCTGTTTTGTGGAGGGTAAGTACCTGCA |
| TUR | 35,611,736 | 35,611,757 | AGCACCCTTAAGACTTCCCTTC |
| TDF | 35,608,143 | 35,608,165 | TCCTGTTAGTGCCTGGGATAAAG |
| TDP | 35,608,106 | 35,608,132 | TGTCTATCCCTGCTAACTGGTAAGGCA |
| TDR | 35,608,038 | 35,608,058 | TCTGGATGCCAAGTGTCTTAC |
PCR Primers
| Primer | Sequence |
| SU | AGGATGACCAAACATTTCCC |
| SD | GGCTTAGACATTGCAAACTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 128 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13870A-D9 | VGB6 | C57BL/6NTac | |
| 13870A-E11 | VGB6 | C57BL/6NTac | |
