| Gene | Syn3 |
| Chromosome | 10 |
| Deletion Start | 86,467,286 |
| Deletion End | 86,448,872 |
| Deletion Size | 18,415 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,467,019 | 86,467,038 | CACACCCGTGGTTCAGAGAC |
| TUP | 86,466,984 | 86,467,011 | CTTGTTGGTTATCGATGACGCCCATACA |
| TUR | 86,466,939 | 86,466,960 | GAGATAGCCACACCCAGATTCC |
| TDF | 86,449,133 | 86,449,151 | TGCAGTAGAGGCAGAGGAG |
| TDP | 86,449,103 | 86,449,130 | CAAGTCACTGGACAGAACCAGAACTCGA |
| TDR | 86,449,073 | 86,449,095 | GGAGGAAGAGTGGATAGGAAGAC |
PCR Primers
| Primer | Sequence |
| SU | TGTCCTATGAATGCTGGTGG |
| SD | GGTTGCAAATTAGTGGACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13871A-D1 | VGB6 | C57BL/6NTac | |
| 13871A-D8 | VGB6 | C57BL/6NTac | |
| 13871A-E6 | VGB6 | C57BL/6NTac | |
| 13871A-F5 | VGB6 | C57BL/6NTac | |
