| Gene | Tekt2 |
| Chromosome | 4 |
| Deletion Start | 126,324,955 |
| Deletion End | 126,322,187 |
| Deletion Size | 2,769 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,324,716 | 126,324,738 | TTGGTTCTGTCTCTTCCAGATTG |
| TUP | 126,324,682 | 126,324,706 | AACATGACAATAGGACCCGCCTGGC |
| TUR | 126,324,640 | 126,324,657 | TCCAGCGTCTCCTTCCAC |
| TDF | 126,322,993 | 126,323,014 | GGGAGCCCTAGTGTGAGTCTTG |
| TDP | 126,322,967 | 126,322,992 | CCGGTGGAGAACAGTAATGTCTGCCA |
| TDR | 126,322,938 | 126,322,957 | TGCAGCTCGGCTATCTCTTC |
PCR Primers
| Primer | Sequence |
| SU | CCTGCCATCCCTTCAGTAAG |
| SD | TGAACAAGAATGTTGCCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13880A-C11 | VGB6 | C57BL/6NTac | |
| 13880A-H5 | VGB6 | C57BL/6NTac | |
