| Gene | Tgfbrap1 |
| Chromosome | 1 |
| Deletion Start | 43,075,935 |
| Deletion End | 43,049,603 |
| Deletion Size | 26,333 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,075,489 | 43,075,507 | GCGCAGGACTGTCCAAATG |
| TUP | 43,075,463 | 43,075,488 | TTTCTGGTGTATGAGGACCGAGTGCA |
| TUR | 43,075,412 | 43,075,430 | TCCACAGCCACAGCAAGAG |
| TDF | 43,049,802 | 43,049,821 | CAATGCAGTCCGGCTCTCAG |
| TDP | 43,049,772 | 43,049,795 | AGCTTTGCCAGCTCTGCCAGAACC |
| TDR | 43,049,727 | 43,049,747 | CAAGCCCTCCATTTGGGTATC |
PCR Primers
| Primer | Sequence |
| SU | GCCTCCTTCAGAAGTCCCTC |
| SD | GCAGAGGGTTTCAGAGGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13881A-F9 | VGB6 | C57BL/6NTac | |
| 13881B-H3 | VGB6 | C57BL/6NTac | |
| 13881C-A5 | VGB6 | C57BL/6NTac | |
