| Gene | Timm13 |
| Chromosome | 10 |
| Deletion Start | 80,900,740 |
| Deletion End | 80,900,113 |
| Deletion Size | 628 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,900,666 | 80,900,685 | GGGCCATTATGGAGCAGGTG |
| TUP | 80,900,643 | 80,900,665 | AAAGTGCAGATCGCCGTGGCCAA |
| TUR | 80,900,540 | 80,900,560 | TCCGTCATTCTCTGTGGACAC |
| TDF | 80,900,185 | 80,900,204 | GCGCCTACAACTCTCGACTG |
| TDP | 80,900,161 | 80,900,184 | CAGCGGGAACGAGCCAACATGTGA |
| TDR | 80,900,122 | 80,900,142 | TCAGACCATAAGCACGGTGTC |
PCR Primers
| Primer | Sequence |
| SU | GTTCGGGTTTCCTTCCTTTC |
| SD | AGATACCCTGGGAGGTCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 146 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13883A-A3 | VGB6 | C57BL/6NTac | |
| 13883A-A9 | VGB6 | C57BL/6NTac | |
| 13883A-B8 | VGB6 | C57BL/6NTac | |
| 13883A-H9 | VGB6 | C57BL/6NTac | |
| 13883A-E7 | VGB6 | C57BL/6NTac | |
