| Gene | Tinf2 |
| Chromosome | 14 |
| Deletion Start | 55,681,578 |
| Deletion End | 55,679,307 |
| Deletion Size | 2,272 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,680,661 | 55,680,679 | CGGGCTCGTTCATCACTTC |
| TUP | 55,680,632 | 55,680,659 | TCGGTTGCTTTGCACCAGTATGGTATGG |
| TUR | 55,680,592 | 55,680,613 | TCTCCGCTGATCCTCTTACCTG |
| TDF | 55,679,960 | 55,679,979 | CCTGCCTGCTTCCCTTCTTC |
| TDP | 55,679,931 | 55,679,958 | CCCAACCTTTCCTTTGCCTTCACCTCAG |
| TDR | 55,679,884 | 55,679,901 | GAGGCGGTGATGAGTGTC |
PCR Primers
| Primer | Sequence |
| SU | GTACGTCACTTTCACAGTGC |
| SD | TGGCATAAGGACAGTTTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13884A-F1 | VGB6 | C57BL/6NTac | |
| 13884A-C10 | VGB6 | C57BL/6NTac | |
| 13884A-E5 | VGB6 | C57BL/6NTac | |
| 13884A-A9 | VGB6 | C57BL/6NTac | |
| 13884A-G7 | VGB6 | C57BL/6NTac | |
| 13884A-B11 | VGB6 | C57BL/6NTac | |
