| Gene | Tlcd1 |
| Chromosome | 11 |
| Deletion Start | 78,178,866 |
| Deletion End | 78,180,468 |
| Deletion Size | 1,603 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,179,550 | 78,179,569 | CCACGACACAGTGGACATTG |
| TUP | 78,179,570 | 78,179,597 | TGGTTAGCAAGCAAACTCGAGCTTCTTG |
| TUR | 78,179,623 | 78,179,641 | TCACCGGGTTCTCCCTTAC |
| TDF | 78,180,187 | 78,180,204 | AGGACCCTGGGCACATTC |
| TDP | 78,180,205 | 78,180,228 | TTGTTGGCCATCCTGCTCATGCTG |
| TDR | 78,180,259 | 78,180,279 | GCAGAAATCAGAGCGGAGGAG |
PCR Primers
| Primer | Sequence |
| SU | CAACGATGGCGAGGACAG |
| SD | ACAGGTGTTGGTTCTCCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13885A-G9 | VGB6 | C57BL/6NTac | |
| 13885A-A5 | VGB6 | C57BL/6NTac | |
| 13885A-B8 | VGB6 | C57BL/6NTac | |
| 13885A-F9 | VGB6 | C57BL/6NTac | |
| 13885A-F5 | VGB6 | C57BL/6NTac | |
