| Gene | Tm2d2 |
| Chromosome | 8 |
| Deletion Start | 25,017,372 |
| Deletion End | 25,022,807 |
| Deletion Size | 5,436 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,018,283 | 25,018,304 | GTCGCTGGAAACATGACAAAGG |
| TUP | 25,018,309 | 25,018,335 | AACGGCTGAAGAGCACATTTGCTTTCC |
| TUR | 25,018,344 | 25,018,363 | CTTGGGCACAGGCATGGTTG |
| TDF | 25,022,146 | 25,022,168 | TCATTCCAGGGTCAAGTGTCAAG |
| TDP | 25,022,169 | 25,022,194 | CCATTGAGGCCCACACTTAAAGCTGC |
| TDR | 25,022,218 | 25,022,240 | GGCAGGACAGATCAGCAATTTAG |
PCR Primers
| Primer | Sequence |
| SU | GAGACTAGAGAAGGTAGGAG |
| SD | TCATTGGTGCAGCTTCTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13886A-E8 | VGB6 | C57BL/6NTac | |
| 13886A-G6 | VGB6 | C57BL/6NTac | |
