| Gene | Tm4sf5 |
| Chromosome | 11 |
| Deletion Start | 70,505,334 |
| Deletion End | 70,510,907 |
| Deletion Size | 5,574 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,505,717 | 70,505,737 | GGAATGTGGGAAAGGAAGGAC |
| TUP | 70,505,743 | 70,505,770 | TACTAGGTGGGAGTAAGTTCTCTGTGCC |
| TUR | 70,505,772 | 70,505,795 | ATGTTCAGTAGTAGAGGCAAGGAG |
| TDF | 70,510,255 | 70,510,273 | GCGCCATCTACTGCCTGTC |
| TDP | 70,510,283 | 70,510,309 | AGCTGGGCTCCGAATTGGACCCAAATG |
| TDR | 70,510,317 | 70,510,336 | TGGTAGTCCCACTTGTTGTC |
PCR Primers
| Primer | Sequence |
| SU | CAAGGCTTTCTAACCCATCG |
| SD | TCACTGGCACATTAGCTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13888A-A12 | VGB6 | C57BL/6NTac | |
| 13888A-B12 | VGB6 | C57BL/6NTac | |
| 13888A-F6 | VGB6 | C57BL/6NTac | |
| 13888A-G2 | VGB6 | C57BL/6NTac | |
| 13888A-G5 | VGB6 | C57BL/6NTac | |
| 13888A-G9 | VGB6 | C57BL/6NTac | |
