| Gene | Tmem109 |
| Chromosome | 19 |
| Deletion Start | 10,874,475 |
| Deletion End | 10,871,734 |
| Deletion Size | 2,742 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,874,354 | 10,874,373 | CATCGAGAGTTTGCCTCACC |
| TUP | 10,874,327 | 10,874,353 | TGGTCAACAGAAGAAGGAGACCTCAGC |
| TUR | 10,874,269 | 10,874,290 | CCCTAGCCAGGTATCCAACATC |
| TDF | 10,872,649 | 10,872,668 | GCCATCTCATCAGCCATCTC |
| TDP | 10,872,628 | 10,872,648 | TGTGGCCTGCTTTGCCCTGTC |
| TDR | 10,872,606 | 10,872,624 | CAGCAACTGGGCAGCAATC |
PCR Primers
| Primer | Sequence |
| SU | GGCATAGGGTAGTACTTGCTGG |
| SD | ACTTGGGATGGTAGTGTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 63 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13893A-B2 | VGB6 | C57BL/6NTac | |
| 13893A-B8 | VGB6 | C57BL/6NTac | |
| 13893A-C8 | VGB6 | C57BL/6NTac | |
| 13893A-D11 | VGB6 | C57BL/6NTac | |
