| Gene | Tmem127 |
| Chromosome | 2 |
| Deletion Start | 127,248,569 |
| Deletion End | 127,257,305 |
| Deletion Size | 8,737 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,248,856 | 127,248,878 | AACAGATATGAGGCTGGCTGAAG |
| TUP | 127,248,886 | 127,248,912 | AGCCAGAGGCAGTCATTTGTCAAAGGA |
| TUR | 127,248,921 | 127,248,943 | GGCATGAACACCAAGCAAGAATG |
| TDF | 127,257,015 | 127,257,036 | CCAGCAGCAACAGCATAAGAAG |
| TDP | 127,257,039 | 127,257,063 | CCACGGCTCCCAAGTCTATGTCACC |
| TDR | 127,257,078 | 127,257,097 | CAGCTCCTGCCACCAAGTAG |
PCR Primers
| Primer | Sequence |
| SU | ATAACCCTGGTCCAAGTCTC |
| SD | AAGACATGGCCCACAGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13894A-B8 | VGB6 | C57BL/6NTac | |
| 13894A-E12 | VGB6 | C57BL/6NTac | |
