| Gene | Tmem145 |
| Chromosome | 7 |
| Deletion Start | 25,306,167 |
| Deletion End | 25,316,408 |
| Deletion Size | 10,242 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,306,507 | 25,306,526 | TGGTGGTGCTGAAGGGACAG |
| TUP | 25,306,537 | 25,306,559 | TCCACCAAGTGGAGGTTGCCTGG |
| TUR | 25,306,583 | 25,306,602 | TCCTGGGCAATCTCCCAAAC |
| TDF | 25,316,282 | 25,316,303 | TGTAGGAGAAGGCCACAAGAAG |
| TDP | 25,316,305 | 25,316,334 | CGTTGGATTTCCACTATATGGGTTCTGGGA |
| TDR | 25,316,367 | 25,316,388 | GAGGCGACTTAGTGGTTCAGAG |
PCR Primers
| Primer | Sequence |
| SU | CAAAGAAGACCCTTGCTTGC |
| SD | GAATAGGAATTCAGCTCAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13895A-C4 | VGB6 | C57BL/6NTac | |
| 13895B-A11 | VGB6 | C57BL/6NTac | |
