| Gene | Tmem151a |
| Chromosome | 19 |
| Deletion Start | 5,085,369 |
| Deletion End | 5,081,736 |
| Deletion Size | 3,634 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,085,200 | 5,085,222 | GAGAGGGATTGAGGAGGAAAGTC |
| TUP | 5,085,179 | 5,085,199 | CCGGAGCCCCTCCAGCAATGA |
| TUR | 5,085,156 | 5,085,177 | AGAACGCTGTCTCCCCAATGAC |
| TDF | 5,082,509 | 5,082,528 | CGGCTGCGTTTCACCAAGTG |
| TDP | 5,082,484 | 5,082,508 | CTTCAGCTTTGGGAGTGCTGAGGCC |
| TDR | 5,082,425 | 5,082,442 | TCGTCCAGGCCCTCATTG |
PCR Primers
| Primer | Sequence |
| SU | TGAAGTCTGGGTGGGCTTAG |
| SD | AGCCAGCTGTTCAGCTTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13896A-E12 | VGB6 | C57BL/6NTac | |
| 13896A-E2 | VGB6 | C57BL/6NTac | |
| 13896A-D12 | VGB6 | C57BL/6NTac | |
