| Gene | Tmem191c |
| Chromosome | 16 |
| Deletion Start | 17,276,317 |
| Deletion End | 17,278,196 |
| Deletion Size | 1,880 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,277,070 | 17,277,089 | GCTGTGGGCAGGGTTATGTG |
| TUP | 17,277,096 | 17,277,120 | TGGGCAGAGTATCAGCCTTCAAGGC |
| TUR | 17,277,193 | 17,277,211 | CGCCAGCTCCAGATGTTTC |
| TDF | 17,277,490 | 17,277,509 | CGCATCCTCTGCCCTGAATC |
| TDP | 17,277,512 | 17,277,537 | TATTGCCATCCTTCAGGAGCTGCAGG |
| TDR | 17,277,543 | 17,277,560 | AGCTGCTGCCTCCATCAC |
PCR Primers
| Primer | Sequence |
| SU | ATACCTGAATTGGTCGGGC |
| SD | ATGCTGTGAGGGAGGTATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 142 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13899A-A4 | VGB6 | C57BL/6NTac | |
| 13899A-D9 | VGB6 | C57BL/6NTac | |
| 13899A-D10 | VGB6 | C57BL/6NTac | |
| 13899A-G1 | VGB6 | C57BL/6NTac | |
| 13899A-G11 | VGB6 | C57BL/6NTac | |
