| Gene | Tmem202 |
| Chromosome | 9 |
| Deletion Start | 59,525,439 |
| Deletion End | 59,518,987 |
| Deletion Size | 6,453 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,524,831 | 59,524,849 | TGCTACTGGCCTGCACATC |
| TUP | 59,524,796 | 59,524,823 | AACTTGGTGCAGTTCCTGGTGAACAACA |
| TUR | 59,524,764 | 59,524,784 | GGTCCAGAGTCCTGCTTTGAG |
| TDF | 59,519,773 | 59,519,794 | CGGAAGATGCTGATAGGTTGTG |
| TDP | 59,519,739 | 59,519,761 | TTTGGTCCCAGCTGTCAGCTTGC |
| TDR | 59,519,712 | 59,519,730 | GCGCCAGAAACAGGTTGAG |
PCR Primers
| Primer | Sequence |
| SU | GGGTGACCCAAGTTAGAGTCC |
| SD | TTCCTCTGAATTCTCACTACCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13901A-A2 | VGB6 | C57BL/6NTac | |
| 13901A-A4 | VGB6 | C57BL/6NTac | |
| 13901A-B6 | VGB6 | C57BL/6NTac | |
| 13901A-B8 | VGB6 | C57BL/6NTac | |
| 13901A-E10 | VGB6 | C57BL/6NTac | |
| 13901A-G7 | VGB6 | C57BL/6NTac | |
