| Gene | Tmem85 |
| Chromosome | 2 |
| Deletion Start | 112,367,901 |
| Deletion End | 112,363,311 |
| Deletion Size | 4,591 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 112,367,462 | 112,367,482 | TCCCTCTATCCAGTCGGTTAC |
| TUP | 112,367,431 | 112,367,456 | CAAGCAAGTGCCTGATACCAGCGTGC |
| TUR | 112,367,384 | 112,367,408 | CGTCTAATACCTCTGTACCTTCTCC |
| TDF | 112,363,926 | 112,363,949 | TCAAGATGCTGGAGAGTTCAAGTC |
| TDP | 112,363,890 | 112,363,919 | TTCTTCAAGGCTTGGTCTATCTCATTGGGA |
| TDR | 112,363,860 | 112,363,881 | TGTAAACTGCCAATGCCAAACC |
PCR Primers
| Primer | Sequence |
| SU | AGACAGAGCACAAGTGAGAG |
| SD | CATTAAGCATTGTTCTCAACCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13903A-H12 | VGB6 | C57BL/6NTac | |
| 13903A-F1 | VGB6 | C57BL/6NTac | |
| 13903A-H6 | VGB6 | C57BL/6NTac | |
| 13903A-E4 | VGB6 | C57BL/6NTac | |
