| Gene | Tmem93 |
| Chromosome | 11 |
| Deletion Start | 73,176,700 |
| Deletion End | 73,176,292 |
| Deletion Size | 409 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,176,603 | 73,176,624 | ATTACTGCCGGACCTCAGTGTC |
| TUP | 73,176,570 | 73,176,588 | CCACCGCCGGCATCCTCGG |
| TUR | 73,176,527 | 73,176,548 | GGAGGCAAGCAGGTAGAAGATG |
| TDF | 73,176,410 | 73,176,429 | GCCTCTTCACCTACGTCCTC |
| TDP | 73,176,382 | 73,176,407 | TTGGACCTTCCTCTATGGCATGGTGC |
| TDR | 73,176,358 | 73,176,379 | CCCTGCCCTCATTTCAGTAGAC |
PCR Primers
| Primer | Sequence |
| SU | AGCCTCCAGCGCAAGATGG |
| SD | AGTAACACGAACTGTTACCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13904A-A9 | VGB6 | C57BL/6NTac | |
| 13904A-A11 | VGB6 | C57BL/6NTac | |
| 13904A-B7 | VGB6 | C57BL/6NTac | |
| 13904A-B8 | VGB6 | C57BL/6NTac | |
| 13904A-B11 | VGB6 | C57BL/6NTac | |
| 13904A-C5 | VGB6 | C57BL/6NTac | |
